马慧敏(MA HUIMIN)

马慧敏,博士,从事环境污染物的早期健康效应研究,研究环境中的重金属、大气细颗粒物(包括PM2.5和PM0.1)等对人体健康的影响。利用环境有机地球化学、流行病学、和毒理学等方法,筛选重金属暴露对儿童智力发育的影响及其特异性生物标志物,探索重金属对儿童神经发育的快速评估系统;筛选和鉴定大气颗粒物中的有效毒性种类,鉴定特异性生物标志物,阐述PM中新污染物对人体的毒作用模式。

针对环境中重金属的污染问题,结合流行病学数据和体外验证结果,提出砷、汞、铅和镉四种重金属低浓度长期暴露的情况下,低于国家血铅阈值(100 μg/L)的血铅仍然是诱导儿童智力降低的主要因素,筛选出2个印记基因,作为血铅诱导儿童智力降低的生物标志物,并进一步探索评估铅对智力影响的快速检测系统。研究发表在环境领域顶级期刊ES&T和EP杂志上,并作为评价多种重金属低浓度暴露下血铅对儿童智力有显著影响的有力数据,被全球环境健康权威数据库美国环保署综合风险信息系统(US EPA IRIS)关注并收录作为各国政府重金属公共卫生管理的决策依据。

筛选鉴定PM2.5/PM0.1样品各组分中的有效毒性物质,依据流行病学和人体细胞实验结果,利用傅里叶变换离子回旋共振质谱仪(FT-ICR MS)、加速器质谱(AMS)、核磁共振(NMR)、制备性液相色谱分离技术、气相液相质谱技术等地球化学方法分析新污染物的组成、结构、来源等信息。初步筛选出PM2.5中来源于生物质燃烧、煤炭燃烧和二次污染源的类腐殖酸类化合物(Huminc-like Substances, HULIS)可能是诱导炎症和凋亡的主要毒性组分,其芳香环数和双键数起关键作用,并筛选鉴定出新化合物的分子式。研究结果发表在环境领域顶级期刊ES&T和EP等杂志上,并选为ES&T当期的内封面。

在国内首次建立HPLC-MS/MS全基因组DNA甲基化和MALDI-TOF-MS/MS分析组蛋白修饰的检测方法,分析了环境中普遍存在的三氯生和苯及其代谢物低浓度暴露诱导的毒性效应,并筛选出了潜在的分子标志物。关于三氯生的文章,被英国知名的文献推荐平台F1000prime推荐为“非常好”的论文,并评价了论文结果对三氯生肝毒性的贡献。

 学习工作简历:

1999-2003:安徽医科大学,生物技术专业, 学士学位;

2003-2009:中国科学院广州地球化学研究所,博士学位;

2007-2008:Jacobs University,German,  Biochemistry, 联合培养博士生;

2009 – 2011:有机地球化学国家重点实验室, 中国科学院广州地球化学研究所,助理研究员;

2011 –至今:有机地球化学国家重点实验室, 中国科学院广州地球化学研究所;副研究员;

2018 – 2019:Lancaster Environmental Center, Lancaster University, Lancaster UK, Visiting Scholar.

主要发表论文:

  • Cong Wan, Shangxia Pan, Lifeng Lin, Jun Li, Guanghui Dong, Kevin C. Jones, Fei Liu, Daochuan Li, Jing Liu, Zhiqiang Yu, Gan Zhang, and Huimin Ma*, DNA Methylation Biomarkers of IQ Reduction are Associated with Long Term Lead Exposure in School Aged Children in Southern China . Environ Sci Technol, 2020, http://dx.doi.org/10.1021/acs.est.0c01696;
  • Yaohui Liang, Liwen Hu, Jun Li, Fei Liu, Kevin C. Jones, Daochuan Li, Jing Liu, Duohong Chen, Jing You, Zhiqiang Yu, Gan Zhang, Guanghui Dong, Huimin Ma*, Short-term personal PM2.5 exposure and change in DNA methylation of imprinted genes: Panel study of healthy young adults in Guangzhou city, China, Environmental Pollution, 2021 (accepted).
  • Huimin Ma, Jun Li, Cong Wan, Yaohui Liang, Yangzhi Mo, Xiangyun Zhang, Guanghui Dong, Liwen Hu, , Xiaowen Zeng, Kevin C. Jones, Zhiqiang Yu, Gan Zhang*,Potential chemical components and signaling pathways that induce apoptosis in PM2.5 from 10 major cities in China, Environ Sci Technol. (under reviewed)
  • Huimin Ma, Jun Li, Cong Wan, Yaohui Liang, Xiangyun Zhang, Guanghui Dong, Liwen Hu, Boyi Yang, Xiaowen Zeng, Tao Su, Shaoyou Lu, Shejun Chen, Mahdi Safaei Khorram, Guoying Sheng, Xinming Wang, Bixian Mai, Zhiqiang Yu*, Gan Zhang*, Inflammation response of water-soluble fractions in atmospheric fine particulates: a seasonal observation in 10 large Chinese cities, Environ Sci Technol. 2019, 53(7):3782-3790.
  • Shangxia Pan, Lifeng Lin, Fan Zeng, Jianpeng Zhang, Guanghui Dong, Boyi Yang, You Jing, Shejun Chen, Gan Zhang, Zhiqiang Yu, Guoying Sheng, Huimin Ma*, Effects of lead, cadmium, arsenic, and mercury co-exposure on children’s intelligence quotient in an industrialized area of southern China, Environmental Pollution, 2018, 20(235):47-54.
  • Liudan Zeng, Huimin Ma*, Shangxia Pan, Jing You, Gan Zhang, Zhiqing Yu, Guoying Sheng, Jiamo Fu,LINE-1 gene hypomethylation and p16 gene hypermethylation in HepG2 cells induced by low-dose and long-term Triclosan exposure: The role of hydroxyl group, Toxicology in vitro, 2016,34:35-44.
  • Junjie Hu, Huimin Ma*, Wenbing Zhang*, Zhiqing Yu, Guoying Sheng, Jiamo Fu,Effects of benzene and its metabolites on global DNA methylation in human normal hepatic L02 cells,Environmental Toxicology. 2014, 29:108-116.
  • Huimin Ma*, Liujin Zheng, Yuhua Li, Shaxia Pan, Zhiqiang Yu, Gan Zhang, Guoying Sheng, Jiamo Fu,Triclosan Reduces the Levels of Global DNA Methylation in HepG2 Cells,Chemosphere, 2013,90:1023-1029.
  • Junjie Hu, Wenbing Zhang*, Huimin Ma*, Yunmei Cai, Guoying Sheng, Jiamo Fu.Simultaneous determination of 8-hydroxy-2 ‘-deoxyguanosine and 5-methyl-2 ‘-deoxycytidine in DNA sample by highperformance liquid chromatography/positive electrospray ionization tandem mass spectrometry. Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences. 2010, 878:2765-2769.
  • Huimin Ma, Wenbing Zhang*, Junjie Hu, Zhiqiang Yu, Yong Chen, Qiong luo, Xiaoliu Shi, Yingying Zhang, Renfang Song, Zhen Zhou, Guoying Sheng and Jiamo Fu, “Analysis of global DNA methylation level in human blood using high-performance liquid chromatography/tandem electrospray ionization mass spectrometry (HPLC-ESI-MS/MS)”, European Journal of Mass Spectrometry, 2009 ,15: 555-561.
  • Huimin Ma, Wenbing Zhang*, Renfang Song, Huayong Chen, Guoying Sheng, Zhen Zhou and Jiamo Fu, “Characterization of methylated and unmethlyated CpG oligodeoxynucleotides by electrospray ionization tandem mass spectrometry”, European Journal of Mass Spectrometry,2005,11:581-589;
  • Yang B, Qian Z, Vaughn M, Nelson E, Dharmage S, Heinrich J, Lin S, Lawrence WR, Ma H, Chen D, Hu L, Zeng X, Xu S, Zhang C, Dong G. Is prehypertension more strongly associated with long-term ambient air pollution exposure than hypertension? Findings from the 33 Communities Chinese Health Study. Environmental Pollution. 2017;229:696-704.
  • 潘尚霞,马慧敏*,林立丰,张建鹏,万聪,环境铅暴露对儿童智力和精神运动功能影响研究,华南预防医学,2017,5:412-417。
  • 曾柳丹,马慧敏,*, 张干, 于志强, 盛国英,傅家谟, BDE-208和BDE-209诱导了LINE-1基因低甲基化和ESR1基因高甲基化,生态毒理学报,2016,2:163-169。
  • 王晶晶、马慧敏*、李宝才*、郑刘进、潘尚霞、黎玉华、盛国英、傅家谟,苯代谢物氢醌对人体肝细胞内DNA甲基转移酶基因和蛋白表达的影响 ,昆明理工大学学报(自然科学版), 2013,38:90-95.
  • 胡俊杰、马慧敏*、张文兵、李林朋、于志强、盛国英、傅家谟,苯急性暴露对Sprague-Dawley大鼠全基因组DNA 甲基化的影响,生态环境学报,2010, 19(12):2902-2905.
  • 马慧敏,张文兵*,施小六,于志强,胡俊杰,宋任芳,盛国英,傅家谟, “人体结肠癌组织样品中基因组DNA甲基化水平的HPLC-ESI-MS/MS检测”,分析测试学报, 2009, 5:292-296.
  • . Philipp Rathert, Arunkumar Dhayalan, Huimin Ma and Albert Jeltsch*, “Specificity of protein lysine methyltransferases and methods for detection of lysine methylation of non-histone proteins” Moecular. BioSystems, 2008,4:1186 – 1190;
  • Renfang Song, Wenbing Zhang, Huayong Chen, Huimin Ma, Yulian Dong, Guoying Sheng, Zhen Zhou and Jiamo Fu*,“Characterization of G-rich and T-rich oligonucleotides using ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry”, European Journal of Mass Spectrometry, 2004,10:705-713。
  • Renfang Song, Wenbing Zhang, Huayong Chen,Huimin Ma,Yulian Dong, Guoying Sheng, Zhen Zhou and Jiamo Fu*, “Comparison of A+T rich oligonucleotides with and without self-complementary sequence using ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry”, European Journal of Mass Spectrometry,2005, 11:83-92
  • Huayong Chen, Wenbing Zhang,Renfang Song,Huimin Ma,Yulian Dong, Guoying Sheng, Zhen Zhou and Jiamo Fu*, “Analysis of DNA methylation by ion-pair reversed-phase high-performance liquid chromatography/tandem electrospray ionization mass spectrometry”, Rapid Communications in Mass Spectrometry , 2004, 18:2773-2778
  • Renfang Song, Wenbing Zhang, Huayong Chen,Huimin MaYulian Dong, Guoying Sheng, Zhen Zhou and Jiamo Fu*, “Site determination of phenyl glycidyl ether-DNA adducts using high-performance liquid chromatography tandem electrospray ionization mass spectrometry”, Rapid Communications in Mass Spectrometry , 2005, 19: 1120-1124
  • Renfang Song, Wenbing Zhang,Huayong Chen,Huimin Ma,Yulian Dong, Guoying Sheng, Zhen Zhou and Jiamo Fu*,“Analysis of SNPs sites in exon 4 of the P53 gene using high-performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry”, European Journal of Mass Spectrometry , 2006, 12: 205-211
  • Renfang Song, Wenbing Zhang, Huayong Chen,Huimin Ma,Yulian Dong, Guoying Sheng, Zhen Zhou and Jiamo Fu*,“Characterization of activation energy distribution for UV irradiation of T-rich oligonucleotide using high-performance liquid chromatography electrospray ionization mass spectrometry tandem mass spectrometry”, European Journal of Mass Spectrometry, 2006, 12 :283-289

主要学术任职:

  • 2019年被聘为 “中国环境诱变学会”的副监事长;
  • 2017年被聘为“中国毒理学会生化与分子毒理专业委员会”委员.

 参与或主持的项目:

  • 国家自然科学面上项目,项目名称:“大气细颗粒物中类腐殖酸类化合物的有效毒性组分的筛选和鉴定”,2021年1月到2024年12月,经费57万,项目号:42077197,课题负责人;
  • 国家重点研发计划国合专项,2018YFE0106900,交通源超细颗粒物暴露与神经退行性疾病风险评估的关键技术及应用,2018YFE0106900 2019-7至2022-6,570万,第三课题负责人;
  • 广东省自然科学基金,面上项目,2019A1515011859, 血铅诱导儿童智力降低的DNA甲基化生物标志物的初步鉴定,2019-10至2022-9, 10万,主持人;
  • 广州市科技计划项目,项目名称:“广州市5中不同来源的类腐殖酸类化合物对人体肺细胞的毒性贡献”,2021年3月-2023年3月,经费:5万,主持人。
  • 国家基金重点项目,项目名称 “大气颗粒物中多环芳香有机质(POM)化学组成与毒性的分布和演化”, 2021年1月到2025年12月, 经费:304万,项目号:42030715,主要参与人员;
  • 广州市科技计划科学研究专项重点项,201707020033,广州不同典型区域大气颗粒物中的毒害有机物及毒性效应,2017-5至2020-12, 200万元,主要参与人员;
  • 国家自然科学青年基金,21007072, HPLC-MS/MS内标法研究典型污染物对人体DNA甲基化的影响,2011-1至2013-12,19 万元,主持;
  • 中国科学院知识创新工程重要方向项目, KZCX2-YW-GJ02, 南亚POPs污染及其跨境迁移趋向, 2010-1至2012-1, 190万元,第二课题主持人;
  • 中国科学院广州地球化学研究所知识创新领域前沿项目,GIGCX-09-01,苯及其代谢物对人体肝脏和骨髓细胞基因的甲基化影响, 2009-11至2011-11, 20万元,主持人。